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Immunology and Infection

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JoVE Journal Immunology and Infection
Coincubation Assay for Quantifying Competitive Interactions between Vibrio fischeri Isolates
 

Coincubation Assay for Quantifying Competitive Interactions between Vibrio fischeri Isolates

Article DOI: 10.3791/59759-v • 07:43 min • July 22nd, 2019
July 22nd, 2019 •
1, 1
1Department of Marine Sciences, University of North Carolina, Chapel Hill

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Resumo
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Bacteria encode diverse mechanisms for engaging in interbacterial competition. Here, we present a culture-based protocol for characterizing competitive interactions between bacterial isolates and how they impact the spatial structure of a mixed population.

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Keywords: Vibrio Fischeri Coincubation Assay Competitive Interactions Antibiotic Resistance Fluorescent Proteins Cell Suspension Optical Density Normalization Differential Tagging Colony Forming Units Fluorescence Microscopy
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