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Journal / Genetics / Transcription Start Site Mapping Using Super-low…
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Genetics

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JoVE Journal Genetics
Transcription Start Site Mapping Using Super-low Input Carrier-CAGE
 

Transcription Start Site Mapping Using Super-low Input Carrier-CAGE

Article DOI: 10.3791/59805-v • 06:59 min • June 26th, 2019
June 26th, 2019 •
1,2, 1,2, 1,2,3
1Institute of Clinical Sciences, Faculty of Medicine, Imperial College London, 2MRC London Institute of Medical Sciences, 3Sars International Centre for Marine Molecular Biology, University of Bergen

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Cap Analysis of Gene Expression (CAGE) is a method for genome-wide quantitative mapping of mRNA 5’ends to capture RNA polymerase II transcription start sites at a single-nucleotide resolution. This work describes a low-input (SLIC-CAGE) protocol for generation of high-quality libraries using nanogram-amounts of total RNA.

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Keywords: Transcription Start Site Mapping SLIC-CAGE Carrier-CAGE RNA Polymerase II Gene Regulation Core Promoter Enhancer Discovery Diagnostic Markers Embryonic Development Reverse Transcription SPRI Magnetic Beads
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