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Inducing and Characterizing Vesicular Steatosis in Differentiated HepaRG Cells
 

Inducing and Characterizing Vesicular Steatosis in Differentiated HepaRG Cells

Article DOI: 10.3791/59843-v • 09:15 min • July 18th, 2019
July 18th, 2019 •
1,2, 3, 3, 3, 2,4, 2,3,5,6, 1,3
1Department of Molecular Medicine, Sapienza University, 2Department of Internal Medicine - DMISM, Sapienza University, 3Center for Life Nano Science@Sapienza, Istituto Italiano di Tecnologia, 4Pasteur Institute Italy-Fondazione Cenci Bolognetti, 5INSERM U1052-Cancer Research Center of Lyon, 6Department of Hepatology, Croix Rousse Hospital, Hospices Civils de Lyon

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In this study, we describe a detailed protocol for inducing liver vesicular steatosis in differentiated HepaRG cells with the fatty acid salt sodium oleate and employ methods for detection and quantification of lipid accumulation, including coherent anti-Stokes Raman scattering (CARS) microscopy, cytofluorimetric analysis, Oil red O staining, and qPCR. 

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Keywords: Vesicular Steatosis HepaRG Cells Differentiation Lipid Accumulation In Vitro Model Hepatocytes Cryopreservation Cell Culture Proliferation Confluency Trypsin Freezing Medium PBS Differentiation Medium Oleate Treatment
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