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JoVE Journal Genetics
Modulation of Tau Subcellular Localization as a Tool to Investigate the Expression of Disease-related Genes
 

Modulation of Tau Subcellular Localization as a Tool to Investigate the Expression of Disease-related Genes

Article DOI: 10.3791/59988-v • 09:12 min • December 20th, 2019
December 20th, 2019 •
1, 1, 1, 1, 1, 1, 1
1Laboratory of Biology, BIO@SNS, Scuola Normale Superiore

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Tau is a neuronal protein present both in the cytoplasm, where it binds microtubules, and in the nucleus, where it exerts unconventional functions including the modulation of Alzheimer's disease-related genes. Here, we describe a method to investigate the function of nuclear Tau while excluding any interferences coming from cytoplasmic Tau.

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Keywords: Tau Subcellular Localization Nuclear Function SH-SY5Y Transfection Tau-NLS Tau-NES Western Blot Protein Extraction Lysis Buffer
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