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Single Cell Durotaxis Assay for Assessing Mechanical Control of Cellular Movement and Related Signaling Events
 

Single Cell Durotaxis Assay for Assessing Mechanical Control of Cellular Movement and Related Signaling Events

Article DOI: 10.3791/59995-v • 08:30 min • August 27th, 2019
August 27th, 2019 •
1,2,3, 1,2,3, 1,2,3, 1,2,3
1Department of Pharmacology, University of Vermont Larner College of Medicine, 2University of Vermont Cancer Center, 3Department of Molecular Physiology and Biophysics, University of Vermont Larner College of Medicine

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Mechanical forces are important for controlling cell migration. This protocol demonstrates the use of elastic hydrogels that can be deformed using a glass micropipette and a micromanipulator to stimulate cells with a local stiffness gradient to elicit changes in cell structure and migration.

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Single Cell Durotaxis Assay Mechanical Control Cellular Movement Signaling Events Microenvironment Experimental Approaches Mechanical Stimulation Dynamic Manipulation Real Time Cell Migration Behavior Sub-cellular Signaling Events Chorono-1 Glass Surface Cover Slip Bind Silane Working Solution Fluorescent Micro-bead Deposition Sonication Plasma Treatment
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