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Specific Labeling of Mitochondrial Nucleoids for Time-lapse Structured Illumination Microscopy
 
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Specific Labeling of Mitochondrial Nucleoids for Time-lapse Structured Illumination Microscopy

Article DOI: 10.3791/60003-v • 07:53 min • June 4th, 2020
June 4th, 2020 •
1, 1, 1
1Imaging Facility, Max Planck Institute of Immunobiology and Epigenetics

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The protocol describes specific labeling of mitochondrial nucleoids with a commercially available DNA gel stain, acquisition of time lapse series of live labeled cells by super-resolution structured illumination microscopy (SR-SIM), and automatic tracking of nucleoid motion.

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Specific Labeling Mitochondrial Nucleoids Time-lapse Structured Illumination Microscopy Fluorescent Dye High Resolution Non-transfectable Cells Preferential Nucleoid Staining SYBR Gold Total Cellular DNA HeLa Cells Petri Dish Culture Medium Labeling Solution Live Imaging Stage Top Incubator Temperature
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