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Journal / Biochemistry / 用于筛选小分子抑制剂的NADH耦合ATPase分析的半高通量自适应…
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用于筛选小分子抑制剂的NADH耦合ATPase分析的半高通量自适应
 
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用于筛选小分子抑制剂的NADH耦合ATPase分析的半高通量自适应

Article DOI: 10.3791/60017-v • 10:28 min • August 17th, 2019
August 17th, 2019 •
1,2, 1,2, 3, 2, 1,2
1Department of Molecular Medicine, The Scripps Research Institute, 2Department of Neuroscience, The Scripps Research Institute, 3Laboratory of Molecular Physiology, NHLBI, National Institutes of Health

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一种烟酰胺腺苷二核苷酸（NADH）与ATPase的检测已应用于小分子肌苷抑制剂的半高通量筛选。此动力学测定以 384 孔微孔板格式运行，总反应体积仅为每孔 20 μL。该平台应适用于几乎任何ADP生产酶。

Tags

生物化学， 问题 150， ATPase 测定， NADH， 荧光， 半高通量筛选， 抑制常数， 肌苷
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