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Immunology and Infection

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JoVE Journal Immunology and Infection
Purification of Human S100A12 and Its Ion-induced Oligomers for Immune Cell Stimulation
 

Purification of Human S100A12 and Its Ion-induced Oligomers for Immune Cell Stimulation

Article DOI: 10.3791/60065-v • 12:55 min • September 29th, 2019
September 29th, 2019 •
1, 1, 1
1Department of Pediatric Rheumatology and Immunology, University Children's Hospital

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This protocol describes a purification method for recombinant tag-free calcium binding protein S100A12 and its ion-induced oligomers for human monocyte stimulation assays.

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