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Immunology and Infection

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JoVE Journal Immunology and Infection
Highly Efficient Transfection of Primary Macrophages with In Vitro Transcribed mRNA
 

Highly Efficient Transfection of Primary Macrophages with In Vitro Transcribed mRNA

Article DOI: 10.3791/60143-v • 06:46 min • November 9th, 2019
November 9th, 2019 •
1, 1, 1, 1,2,3,4, 1
1Institute for Medical Microbiology, Immunology and Hygiene, Faculty of Medicine and University Hospital Cologne, University of Cologne, 2Center for Molecular Medicine Cologne, 3Cologne Cluster of Excellence on Cellular Stress Responses in Aging-associated Diseases (CECAD), 4German Center for Infection Research (DZIF)

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Macrophages, especially primary macrophages, are challenging to transfect as they specialize in detecting molecules of non-self origin. We describe a protocol that allows highly efficient transfection of primary macrophages with mRNA generated from DNA templates such as plasmids.

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Keywords: Transfection Primary Macrophages In Vitro Transcribed MRNA RNA Transcription PolyA Tailing Dephosphorylation MRNA Purification Denaturing Agarose Gel
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