Waiting
Processando Login

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Oligomerization Dynamics of Cell Surface…
JoVE Journal
Biology

É necessário ter uma assinatura JoVE para assistir este Faça login ou comece sua avaliação gratuita.

JoVE Journal Biology
Oligomerization Dynamics of Cell Surface Receptors in Living Cells by Total Internal Reflection Fluorescence Microscopy Combined with Number and Brightness Analysis
 

Oligomerization Dynamics of Cell Surface Receptors in Living Cells by Total Internal Reflection Fluorescence Microscopy Combined with Number and Brightness Analysis

Article DOI: 10.3791/60398-v • 10:43 min • November 6th, 2019
November 6th, 2019 •
1, 2, 1, 3, 1,2
1Unit of Microscopy and Dynamic Imaging, Centro Nacional de Investigaciones Cardiovasculares (CNIC), Madrid, Spain, 2Centro di Imaging Sperimentale, Ospedale San Raffaele, Milan, Italy, 3Unit of Gynecological Oncology Research, European Institute of Oncology IRCCS, Milan, Italy

Capítulos

Resumo
Automatic Translation

We describe an imaging approach for the determination of the average oligomeric state of mEGFP-tagged-receptor oligomers induced by ligand binding in the plasma membrane of living cells. The protocol is based on Total Internal Reflection Fluorescence (TIRF) microscopy combined with Number and Brightness (N&B) analysis.

Tags

Oligomerization Dynamics Cell Surface Receptors Living Cells Total Internal Reflection Fluorescence Microscopy Number And Brightness Analysis Receptor Clustering TIRF Microscopy FGFR1-mEGFP Molecules Plasma Membrane Stimulated Receptor Clustering Spatial Temporal Organization Fluorescence Fluctuation Methods Stoichiometrically Labeled Intracellular Compartments Monomeric Form Of Fluorophore
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter