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JoVE Journal Developmental Biology
Capturing the Cardiac Injury Response of Targeted Cell Populations via Cleared Heart Three-Dimensional Imaging
 

Capturing the Cardiac Injury Response of Targeted Cell Populations via Cleared Heart Three-Dimensional Imaging

Article DOI: 10.3791/60482-v • 08:14 min • March 17th, 2020
March 17th, 2020 •
1, 1, 1
1Department of Cell and Regenerative Biology, University of Wisconsin-Madison School of Medicine and Public Health

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Cardiomyocyte proliferation following injury is a dynamic process that requires a symphony of extracellular cues from non-myocyte cell populations. Utilizing lineage tracing, passive CLARITY, and three-dimensional whole-mount confocal microscopy techniques, we can analyze the influence of a variety of cell types on cardiac repair and regeneration.

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Keywords: Cardiac Injury Cell Populations Three-dimensional Imaging Heart Regeneration LAD Occlusion Myocardial Infarction Tissue Clearing Fluorescent Staining Microscopy
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