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JoVE Journal Developmental Biology
Isolering, kultur och adipogenic Induktion av neurala Crest Original Fett-Härledda stamceller från periaorektisk fettvävnad
 
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Isolering, kultur och adipogenic Induktion av neurala Crest Original Fett-Härledda stamceller från periaorektisk fettvävnad

Article DOI: 10.3791/60691-v • 08:31 min • March 2nd, 2020
March 2nd, 2020 •
*1, *2, 3, 3, 4, 5, 4, 5, 1
1Department of Cardiology, Shanghai Chest Hospital, Shanghai Jiao Tong University, 2Shanghai Institute of Nutrition and Health, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, 3Shanghai Key Laboratory of Hypertension, Shanghai Institute of Hypertension, Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, 4Department of Cardiology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, 5Department of Cardiology, Shanghai Key Laboratory of Clinical Geriatric Medicine, Huadong Hospital Affiliated to Fudan University
* These authors contributed equally

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Vi presenterar ett protokoll för isolering, kultur och adipogenic induktion av neurala crest härledda fett-härledda stamceller (NCADSCs) från periaortic fettvävnad wnt-1 Cre+/-; Rosa26RFP/+ möss. NCADSCs kan vara en lättillgänglig källa till ADSCs för modellering adipogenesis eller lipogenesis in vitro.

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Utvecklingsbiologi Utgåva 157 neurala crest cell Wnt-1 mus periaortic fettvävnad fett-härledda stromal celler stromal vaskulär fraktion cellkultur adipogenic induktion
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