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Journal / Biochemistry / Fully Processed Recombinant KRAS4b: Isolating and …
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Biochemistry

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JoVE Journal Biochemistry
Fully Processed Recombinant KRAS4b
 

Fully Processed Recombinant KRAS4b: Isolating and Characterizing the Farnesylated and Methylated Protein

Article DOI: 10.3791/60703-v • 07:08 min • January 16th, 2020
January 16th, 2020 •
1, 1, 1, 1, 1, 1, 1
1NCI RAS Initiative, Cancer Research Technology Program, Frederick National Laboratory for Cancer Research

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Prenylation is an important modification on peripheral membrane binding proteins. Insect cells can be manipulated to produce farnesylated and carboxymethylated KRAS4b in quantities that enable biophysical measurements of protein-protein and protein-lipid interactions

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Keywords: KRAS4b Farnesylation Methylation Recombinant Protein Protein Purification Cation Exchange Chromatography Baculovirus Expression Cancer Biophysical Experiments
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