Waiting
Processando Login

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Developmental Biology / Simple Lithography-Free Single Cell…
JoVE Journal
Developmental Biology

É necessário ter uma assinatura JoVE para assistir este Faça login ou comece sua avaliação gratuita.

JoVE Journal Developmental Biology
Simple Lithography-Free Single Cell Micropatterning using Laser-Cut Stencils
 

Simple Lithography-Free Single Cell Micropatterning using Laser-Cut Stencils

Article DOI: 10.3791/60888-v • 08:59 min • April 3rd, 2020
April 3rd, 2020 •
*1,2,3, *1,2,3, *1,2,3, 1,2,3, 1,2,3, 1,2,3, 1,2,3, 1,2,3,4,5
1Stanford Cardiovascular Institute, Stanford University School of Medicine, 2Department of Medicine, Division of Cardiovascular Medicine, Stanford University, 3Institute for Stem Cell Biology and Regenerative Medicine, Stanford University, 4Department of Medicine III, University Hospital Heidelberg, 5German Centre for Cardiovascular Research (DZHK), Partner Site Heidelberg/Mannheim
* These authors contributed equally

Capítulos

Resumo
Automatic Translation

This protocol introduces a lithography-free micropatterning method that is simple and accessible to those with a limited bioengineering background. This method utilizes customized laser-cut stencils to micropattern extracellular matrix proteins in a shape of interest for modulating cell morphologies. The procedure for micropatterning is demonstrated using induced pluripotent stem cell derived cardiomyocytes.

Tags

Keywords: Single Cell Micropatterning Laser-cut Stencils Lithography-free Polyacrylamide Hydrogel Basement Membrane Matrix UV Cross-linking Cell Shape Mechanotransduction High-throughput Drug Screening Disease Modeling
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter