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JoVE Journal Genetics
Characterization of In Vitro Differentiation of Human Primary Keratinocytes by RNA-Seq Analysis
 

Characterization of In Vitro Differentiation of Human Primary Keratinocytes by RNA-Seq Analysis

Article DOI: 10.3791/60905-v • 07:29 min • May 16th, 2020
May 16th, 2020 •
*1, *1, 2, 1,3
1Department of Molecular Developmental Biology, Faculty of Science, Radboud Institute for Molecular Life Sciences, Radboud University, Nijmegen, The Netherlands, 2Department of Dermatology, Radboud University Medical Center, Radboud Institute for Molecular Life Sciences, Nijmegen, The Netherlands, 3Department of Human Genetics, Radboud University Medical Center, Nijmegen, The Netherlands
* These authors contributed equally

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Presented here is a stepwise procedure for in vitro differentiation of human primary keratinocytes by contact inhibition followed by characterization at the molecular level by RNA-seq analysis.

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Keywords: In Vitro Keratinocyte Differentiation RNA-seq 2D Monolayer Proliferation Medium Differentiation Medium Cell Seeding Density RNA Isolation CDNA Conversion Library Preparation Next-generation Sequencing Read Mapping DESeq2 RLD Normalization VST Normalization
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