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JoVE Journal Neuroscience
Generation of Oligodendrocytes and Oligodendrocyte-Conditioned Medium for Co-Culture Experiments
 

Generation of Oligodendrocytes and Oligodendrocyte-Conditioned Medium for Co-Culture Experiments

Article DOI: 10.3791/60912-v • 09:05 min • February 9th, 2020
February 9th, 2020 •
1, 1,2, 1,3, 1, 1,2, 1
1Sorbonne Université, Inserm, CNRS, UMR7225, Institut du Cerveau et de la Moelle épinière, ICM, 2Assistance Publique-Hôpitaux de Paris, GH Pitié Salpêtrière, 3Ecole Supérieure des Techniques de Biologie Appliquée

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Herein, we display an efficient method for the purification of oligodendrocytes and production of oligodendrocyte-conditioned medium that can be used for co-culture experiments.

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Keywords: Oligodendrocytes Oligodendrocyte-conditioned Medium Co-culture Multiple Sclerosis Myelination Cell Culture Cerebral Cortices Enzymatic Digestion Tissue Dissociation Cell Filtration
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