É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
1Center for Orthopedic Research and Translational Sciences, Department of Orthopedics and Rehabilitation,Pennsylvania State College of Medicine, 2Department of Biochemistry and Molecular Biology,Pennsylvania State College of Medicine, 3Department of Pharmacology,Pennsylvania State College of Medicine
Capítulos
- 00:04Introduction
- 01:13Histomorphometric Analysis Setup
- 01:59Total, Calcified, and Uncalcified Cartilage Area Measurements
- 02:47Chondrocyte Number and Phenotype Evaluation
- 03:17Tibial Articular Surface Perimeter Measurement
- 03:47Subchondral Bone and Marrow Space Area Measurement
- 04:30Anterior and Posterior Osteophyte Area and Synovial Thickness Measurement
- 05:21Results: Representative Histomorphometric Osteoarthritis (OA) Evaluation in Destabilization of the Medial Meniscus (DMM) and Sham Mice
- 07:06Conclusion
Le protocole actuel établit une méthode rigoureuse et reproductible pour la quantification des changements articulaires morphologiques qui accompagnent l’arthrose. L’application de ce protocole peut être utile en surveillant la progression de la maladie et en évaluant les interventions thérapeutiques dans l’arthrose.
