É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
1Center for Orthopedic Research and Translational Sciences, Department of Orthopedics and Rehabilitation,Pennsylvania State College of Medicine, 2Department of Biochemistry and Molecular Biology,Pennsylvania State College of Medicine, 3Department of Pharmacology,Pennsylvania State College of Medicine
Capítulos
- 00:04Introduction
- 01:13Histomorphometric Analysis Setup
- 01:59Total, Calcified, and Uncalcified Cartilage Area Measurements
- 02:47Chondrocyte Number and Phenotype Evaluation
- 03:17Tibial Articular Surface Perimeter Measurement
- 03:47Subchondral Bone and Marrow Space Area Measurement
- 04:30Anterior and Posterior Osteophyte Area and Synovial Thickness Measurement
- 05:21Results: Representative Histomorphometric Osteoarthritis (OA) Evaluation in Destabilization of the Medial Meniscus (DMM) and Sham Mice
- 07:06Conclusion
Действующий протокол устанавливает строгий и воспроизводимый метод количественной оценки морфологических изменений суставов, которые сопровождают остеоартрит. Применение этого протокола может быть полезным в мониторинге прогрессирования заболевания и оценке терапевтических вмешательств при остеоартрите.
