Waiting
Processando Login

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / Assessment of Human Natural Killer Cell Events…
JoVE Journal
Immunology and Infection

This Conteúdo is Open Access.

JoVE Journal Immunology and Infection
Assessment of Human Natural Killer Cell Events Driven by FcγRIIIa Engagement in the Presence of Therapeutic Antibodies
 

Assessment of Human Natural Killer Cell Events Driven by FcγRIIIa Engagement in the Presence of Therapeutic Antibodies

Article DOI: 10.3791/61144-v • 09:54 min • May 22nd, 2020
May 22nd, 2020 •
1, 2, 1, 2, 1, 1, 1
1Department of Research and Development, iQ Biosciences, 2Department of Cellular Products, iQ Biosciences

Capítulos

Resumo
Automatic Translation

A protocol for studying FcγRIIIa-driven events by therapeutic antibodies in human natural killer cells is described here. This artificial stimulation platform permits the interrogation of downstream effector functions such as degranulation, chemokine/cytokine production, and signaling pathways mediated by the FcγRIIIa and Fc portions of antibodies involved in binding.

Tags

Assessment Human Natural Killer Cell FcγRIIIa Engagement Therapeutic Antibodies Cellular Molecular Mechanism FC Gamma R3A Driven Events Target Cells Refrigerated Micro Centrifuge Resuspended Natural Killer Cells Rituximab Antibody Concentration Incubation Anti-human Kappa Light Chain Antibody Media Solution Antibody Solution Warm-up Centrifugation Washing Steps Cell Activation
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter