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Journal / Genetics / Metylerad RNA immunoprecipitation assay att…
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Genetics

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JoVE Journal Genetics
Metylerad RNA immunoprecipitation assay att studera m5C modifiering i Arabidopsis
 
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Metylerad RNA immunoprecipitation assay att studera m5C modifiering i Arabidopsis

Article DOI: 10.3791/61231-v • 08:50 min • May 14th, 2020
May 14th, 2020 •
*1, *2, 3, 1
1Max Planck Institute of Molecular Plant Physiology, 2Department of Molecular Medicine, Medical School, University of Padua, 3Biologie de l'Ecole Normale Supérieure (IBENS), Paris, France
* These authors contributed equally

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Den metylerade RNA immunoprecipitation assay är en antikroppsbaserad metod som används för att berika för metylerade RNA fragment. Tillsammans med djup sekvensering leder det till identifiering av transkriptioner som bär m5 C-modifieringen.

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Genetik utgåva 159 RNA-metylering 5-metylcytosin (m5C) immunprecipitation (IP) Arabidopsis epitranscriptomics mRNA RNA-sekvensering
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