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JoVE Journal Biochemistry
Rapid and Cost-Effective RNA Extraction of Rat Pancreatic Tissue
 

Rapid and Cost-Effective RNA Extraction of Rat Pancreatic Tissue

Article DOI: 10.3791/61255-v • 10:12 min • September 19th, 2020
September 19th, 2020 •
1,2, 3, 4, 1,5
1Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, 2Endocrinology and Metabolism Research Center, Nemazee Hospital, Shiraz University of Medical Sciences, 3English Language Department, Faculty of Paramedical Sciences, Shiraz University of Medical Sciences, 4Department of e-Learning in Medical Sciences, Virtual School and Center of Excellence in e-Learning, Shiraz University of Medical Sciences, 5Autophagy Research Center, School of Medicine, Shiraz University of Medical Sciences

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The purity and integrity of the isolated RNA is a vital step in RNA dependent assays. Here, we present a practical, rapid, and inexpensive method to extract RNA from a small quantity of undamaged pancreatic tissue.

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RNA Extraction Rat Pancreatic Tissue Rapid Method Cost-effective Method Autolysis RNA Stabilization Reagent Surgery Instruments Ketamine-xylazine Injection Sterilization Incision Abdominal Organs Pancreas Tissue
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