Waiting
Processando Login

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / Spectrophotometric Screening for Potential…
JoVE Journal
Biochemistry
Author Produced

This Conteúdo is Open Access.

JoVE Journal Biochemistry
Spectrophotometric Screening for Potential Inhibitors of Cytosolic Glutathione S-Transferases
 

Spectrophotometric Screening for Potential Inhibitors of Cytosolic Glutathione S-Transferases

Article DOI: 10.3791/61347-v • 14:57 min • October 10th, 2020
October 10th, 2020 •
1,2, *1,3, *1,3
1Research platform of Pediatric Onco-Hematology, Department of Paediatrics, Gynaecology and Obstetrics, Faculty of Medicine, University of Geneva, 2Section of Biology, Faculty of Science, University of Geneva, 3Onco-Hematology Unit, Department of Women, Children-Adolescents, University Hospitals of Geneva
* These authors contributed equally

Capítulos

Resumo
Automatic Translation

Glutathione S-transferases (GSTs) are detoxification enzymes involved in the metabolism of numerous chemotherapeutic drugs. Overexpression of GSTs is correlated with cancer chemotherapy resistance. One way to counter this phenotype is to use inhibitors. This protocol describes a method using a spectrophotometric assay to screen for potential GST inhibitors.

Tags

Keywords: Spectrophotometric Screening Cytosolic Glutathione S-transferases GST Inhibitors Enzymatic Activity Inhibitory Concentration Mode Of Inhibition CDNB GSH PBS
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter