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Examen espectrofotométrico para posibles inhibidores de la citosola glutatión S-transferasas
This content is Free Access.
1Research platform of Pediatric Onco-Hematology, Department of Paediatrics, Gynaecology and Obstetrics, Faculty of Medicine,University of Geneva, 2Section of Biology, Faculty of Science,University of Geneva, 3Onco-Hematology Unit, Department of Women, Children-Adolescents,University Hospitals of Geneva
Capítulos
- 00:00Introduction
- 00:56Preparation of the Enzymatic Solution
- 03:10Michaelis-Menten Constant of CDNB with GST
- 04:42Absorbance of Potential GST Inhibitors
- 06:08Determination of the IC50
- 07:49Determination of the Type of Inhibition and Ki
- 09:46Results: Testing the Potential of Curcumin as a GST Inhibitor
- 13:56Conclusion
Glutatión S-transferasas (GST) son enzimas de desintoxicación implicadas en el metabolismo de numerosos fármacos quimioterápticos. La sobreexpresión de los GST está correlacionada con la resistencia a la quimioterapia contra el cáncer. Una manera de contrarrestar este fenotipo es usar inhibidores. Este protocolo describe un método que utiliza un ensayo espectrofotométrico para detectar posibles inhibidores del GST.
