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Immunology and Infection

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JoVE Journal Immunology and Infection
Live-Cell Forward Genetic Approach to Identify and Isolate Developmental Mutants in Chlamydia trachomatis
 

Live-Cell Forward Genetic Approach to Identify and Isolate Developmental Mutants in Chlamydia trachomatis

Article DOI: 10.3791/61365-v • 10:32 min • June 10th, 2020
June 10th, 2020 •
1, 1, 1
1Department of Biological Sciences, University of Idaho

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This protocol utilizes fluorescent promoter-reporters, live-cell microscopy, and individual inclusion extraction in a directed forward genetic approach to identify and isolate developmental mutants of Chlamydia trachomatis.

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Live-cell Forward Genetic Approach Identify Developmental Mutants Chlamydia Trachomatis Generation And Isolation Altered Developmental Profiles Genes Regulation Track Chlamydia Cell Type Development Mutagenize Chlamydia Trachomatis Reporter Chlamydial Stock Reporter Plasma Centrifugation Sonication EMS Metabolism Solution Mutagenesis Carcinogen Wholesale Culture Imaging And Isolation
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