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Immunology and Infection

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JoVE Journal Immunology and Infection
Using Fluorescent Proteins to Visualize and Quantitate Chlamydia Vacuole Growth Dynamics in Living Cells
 

Using Fluorescent Proteins to Visualize and Quantitate Chlamydia Vacuole Growth Dynamics in Living Cells

Article DOI: 10.3791/51131-v • 07:42 min • October 13th, 2015
October 13th, 2015 •
1,2, 2, 1
1Division of Allergy and Infectious Diseases, Department of Medicine, University of Washington, 2Program in Infectious Diseases, School of Public Health, University of California at Berkeley

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Resumo
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A live cell fluorescent protein based method for illuminating cellular vacuoles (inclusions) containing Chlamydia is described. This strategy enables rapid, automated determination of Chlamydia infectivity in samples and can be used to quantitatively investigate inclusion growth dynamics.

Tags

Fluorescent Proteins Chlamydia Vacuole Growth Dynamics Living Cells Obligate Intracellular Bacterium Chlamydia Elicits Global Public Health Sexually Transmitted Infection Preventable Blindness Host Cell Signaling Novel Vacuolar Compartment Inclusion Developmental Growth Effector Proteins Cytosolic Face Vacuole Membrane Host Cytosol Visualizing Intracellular Compartment Reverse-imaging Strategy GFP Expressing Host Cells MCherry Expressing Host Cells Antibody-based Enumeration Methods
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