JoVE Journal
Genetics
Genetics
This Conteúdo is Open Access.
Capítulos
Resumo
The CRISPR-Cas9 genome editing system is an easy-to-use genome editor that has been used in model and non-model species. Here we present a protein-based version of this system that was used to introduce a premature stop codon into a mating gene of a non-model filamentous ascomycete fungus.