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JoVE Journal Bioengineering
Segmenting Growth of Endothelial Cells in 6-Well Plates on an Orbital Shaker for Mechanobiological Studies
 

Segmenting Growth of Endothelial Cells in 6-Well Plates on an Orbital Shaker for Mechanobiological Studies

Article DOI: 10.3791/61817-v • 12:28 min • June 3rd, 2021
June 3rd, 2021 •
*1,2, *1, 1, 2,3,4, 1
1Department of Bioengineering, Imperial College London, 2Institute of Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR), 3Lee Kong Chian School of Medicine, Nanyang Technological University Singapore, 4Singapore Eye Research Institute, The Academia
* These authors contributed equally

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Resumo
Automatic Translation

This protocol describes a coating method to restrict endothelial cell growth to a specific region of a 6-well plate for shear stress application using the orbital shaker model.

Tags

Endothelial Cells Growth 6-well Plates Orbital Shaker Mechanobiological Studies Coating Method Specific Region Sheer Stress Application Flow Effects Atherosclerosis Protective Flow Cell Behavior Mediators Swirling Medium Shear Stresses Cell Adhesion Stainless Steel Module
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