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Journal / Medicine / 从人内脏脂肪组织中分离出活的脂肪细胞和基质血管组分，适用于 RNA…
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从人内脏脂肪组织中分离出活的脂肪细胞和基质血管组分，适用于 RNA 分析和巨噬细胞表型分析
 
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从人内脏脂肪组织中分离出活的脂肪细胞和基质血管组分，适用于 RNA 分析和巨噬细胞表型分析

Article DOI: 10.3791/61884-v • 06:22 min • October 27th, 2020
October 27th, 2020 •
1, 2, 3, 4, 5, 6, 2, 7, 8
1Research Division, Instituto Nacional de Perinatologia, 2Department of Immunobiochemistry, Instituto Nacional de Perinatologia, 3Department of Obstetrics, Instituto Nacional de Perinatologia, 4Department of Academic Programs and Continuing Education, Instituto Nacional de Perinatologia, 5Department of Infectology and Immunology, Instituto Nacional de Perinatologia, 6Clinical Research Branch, Instituto Nacional de Perinatologia, 7Department of Nutrition and Bioprogramming, Instituto Nacional de Perinatologia, 8Department of Human Genetics and Genomics, Instituto Nacional de Perinatologia, Mexico City, Mexico

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该方案提供了一种高效的胶原酶消化方法，用于在单个过程中从人内脏脂肪中分离活脂肪细胞和基质血管部分-SVF细胞，包括从脂肪细胞中获得高质量RNA的方法，以及通过对多种膜结合标记物进行染色以通过流式细胞术分析的SVF-巨噬细胞表型。

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分离活脂肪细胞、基质血管部分、RNA 分析、巨噬细胞表型分析、内脏脂肪组织、代谢器官、成熟脂肪细胞、生物活性分子、免疫过程、脂肪组织的病理生理学、分离步骤、故障排除指南、胶原酶酶酶消化技术、巨噬细胞亚群、基因表达研究、单细胞悬浮液、成熟脂肪细胞 RNA 分离
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