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Laser Doppler Perfusion Imaging in the Mouse Hindlimb
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JoVE Journal Medicina
Laser Doppler Perfusion Imaging in the Mouse Hindlimb

Laser Doppler Perfusion Imaging in the Mouse Hindlimb

DOI:
10.3791/62012-v

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14:45 min

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April 18, 2021

•

,
1Division of Vascular Surgery,University of Washington, 2VA Puget Sound Health Care System

Capítulos

  • 00:00Introduction
  • 02:39Scanner Preparation
  • 03:14Mouse Pre-Scanning Preparation
  • 06:44Scanning
  • 09:52Capturing LDPI Data
  • 11:22Representative Results
  • 12:18Troubleshooting
  • 14:25Conclusion

Summary

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Tadução automática

Here, we present a protocol that demonstrates the technique and necessary controls for Laser Doppler perfusion imaging to measure blood flow in the mouse hindlimb.

Tags

Laser Doppler Perfusion ImagingLDPIMouse HindlimbSkin UlcerationWound HealingPeripheral Arterial DiseaseRheumatologic ConditionsDiabetesBlood FlowIschemiaIschemia ReperfusionMicrosurgical FlapsAnesthetic AgentsSkin PigmentationArteriogenesisAngiogenesisCollateral Artery DevelopmentMicrofill CastingMicro CT

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