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JoVE Journal Biochemistry
Fast Grid Preparation for Time-Resolved Cryo-Electron Microscopy
 

Fast Grid Preparation for Time-Resolved Cryo-Electron Microscopy

Article DOI: 10.3791/62199-v • 10:05 min • November 6th, 2021
November 6th, 2021 •
1, 2, 3, 1
1School of Biomedical Sciences, Faculty of Biological Sciences & Astbury Centre for Structural and Molecular Biology, University of Leeds, 2School of Molecular and Cellular Biology, Faculty of Biological Sciences & Astbury Centre for Structural and Molecular Biology, University of Leeds, 3Department of Physiological Sciences, Eastern Virginia Medical School

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Here, we provide a detailed protocol for the use of a rapid grid making device for both fast grid-making and for rapid mixing and freezing to conduct time-resolved experiments.

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Grid Preparation Time-resolved Cryo-electron Microscopy Rapid Grid Making Setup Leeds Detailed Account Parameters Laboratories Device Description Richmond Protocol Step-by-step Guide Protein Concentration Volume Device Setup Control PC Control Software Syringe Pumps Potentiometer Power Supply Nitrogen Gas Spray Generation Valves Position Air Bubbles Removal
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