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Immunology and Infection

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JoVE Journal Immunology and Infection
Isolating Brown Adipocytes from Murine Interscapular Brown Adipose Tissue for Gene and Protein Expression Analysis
 

Isolating Brown Adipocytes from Murine Interscapular Brown Adipose Tissue for Gene and Protein Expression Analysis

Article DOI: 10.3791/62332-v • 07:07 min • March 12th, 2021
March 12th, 2021 •
1, 1, 1
1Masonic Medical Research Institute

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This study describes a new method of isolating murine brown adipocytes for gene and protein expression analysis.

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Isolating Brown Adipocytes Murine Interscapular Brown Adipose Tissue Gene Expression Analysis Protein Expression Analysis Brown Adipose Tissue Purification Protocol Development Studying Brown Adipose Tissue Biology White Adipocytes Browning Process Amanda (research Assistant) Flask With BAT And Digestion Solution Magnetic Stirrer Incubator Cell Suspension 70 Micrometer Strainer Filter 12% Iodixanol Solution Polystyrene Test Tubes
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