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Analysis of Motility Patterns of Stentor During and After Oral Apparatus Regeneration Using Cell Tracking
 

Analysis of Motility Patterns of Stentor During and After Oral Apparatus Regeneration Using Cell Tracking

Article DOI: 10.3791/62352-v • 07:17 min • April 26th, 2021
April 26th, 2021 •
1, 2, 3, 4, 4
1W. M. Keck Science Department, Scripps, Pitzer, and Claremont McKenna of The Claremont Colleges, 2W. M. Keck Science Department, Pitzer College, 3W. M. Keck Science Department, Scripps College, 4Department of Biochemistry and Biophysics, School of Medicine, University of California at San Francisco

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We present a protocol for the characterization of motility and behavior of a population of hundred micron- to millimeter-sized cells using brightfield microscopy and cell tracking. This assay reveals that Stentor coeruleus transitions through four behaviorally distinct phases when regenerating a lost oral apparatus.

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Keywords: Stentor Coeruleus Motility Patterns Oral Apparatus Regeneration Cell Tracking Unicellular Regeneration Functional Regeneration Synchronized Regeneration Motility Quantification Silicone Spacer Sample Chambers 10% Sucrose 2% Urea Time-lapse Microscopy Image Calibration Cell Tracking Scripts
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