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Uptake of Fluorescent Labeled Small Extracellular Vesicles In Vitro and in Spinal Cord
 

Uptake of Fluorescent Labeled Small Extracellular Vesicles In Vitro and in Spinal Cord

Article DOI: 10.3791/62537-v • 09:01 min • May 23rd, 2021
May 23rd, 2021 •
1, 1, 1, 1, 1
1Department of Pharmacology & Physiology, Drexel University College of Medicine

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Resumo
Automatic Translation

We describe a protocol to label macrophage-derived small extracellular vesicles with PKH dyes and observe their uptake in vitro and in the spinal cord after intrathecal delivery.

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Keywords: Fluorescent Labeled Small Extracellular Vesicles SEVs Uptake Spinal Cord In Vitro Cell Visualization Neuro-2a Cells Postnatal Pups Cortical Lobes Dissociation Astrocytes Microglia Oligodendrocytes Mechanistic Studies Therapeutic Studies Spinal Disorders Pain Inflammation
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