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Mesoscopic Optical Imaging of Whole Mouse Heart
 

Mesoscopic Optical Imaging of Whole Mouse Heart

Article DOI: 10.3791/62795-v • 08:53 min • October 14th, 2021
October 14th, 2021 •
*1, *1, *1, 1, 1,2, 1,3,4, 1,5, 1,3,4, 1,3,6
1European Laboratory for Non-Linear Spectroscopy, 2Department of Biology, University of Florence, 3National Institute of Optics, National Research Council, 4Department of Physics and Astronomy, University of Florence, 5Department of Neurosciences, Psychology, Drugs and Child Health, University of Florence, 6Institute for Experimental Cardiovascular Medicine, Faculty of Medicine, University of Freiburg
* These authors contributed equally

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Resumo
Automatic Translation

We report a method for mesoscopic reconstruction of the whole mouse heart by combining new advancements in tissue transformation and staining with the development of an axially scanned light-sheet microscope.

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Keywords: Mesoscopic Optical Imaging Whole Mouse Heart Clearing Protocol Mesos PIM Technology Micrometric Resolution 3D Tissue Organization Cardiac Tissue Mouse Heart Hydrogel Solution Clearing Solution Wheat Germ Agglutinin Alexa Fluor 633
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