Waiting
Processando Login

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Transposon-insertion Sequencing as a Tool to…
JoVE Journal
Biology

Este de .

JoVE Journal Biology
Transposon-insertion Sequencing as a Tool to Elucidate Bacterial Colonization Factors in a Burkholderia gladioli Symbiont of Lagria villosa Beetles
 

Transposon-insertion Sequencing as a Tool to Elucidate Bacterial Colonization Factors in a Burkholderia gladioli Symbiont of Lagria villosa Beetles

Article DOI: 10.3791/62843-v • 09:55 min • August 12th, 2021
August 12th, 2021 •
1, 1,2, 1,3
1Department of Evolutionary Ecology, Institute of Organismic and Molecular Evolution, Johannes Gutenberg University, 2Department of Insect Symbiosis, Max Planck Institute for Chemical Ecology, 3Department of Plant and Environmental Sciences, Section for Organismal Biology, University of Copenhagen

Capítulos

Resumo
Automatic Translation

This is an adapted method for identifying candidate insect colonization factors in a Burkholderia beneficial symbiont. The beetle host is infected with a random mutant library generated via transposon mutagenesis, and library complexity after colonization is compared to a control grown in vitro.

Tags

Keywords: Transposon Insertion Sequencing Burkholderia Gladioli Lagria Villosa Beetles Bacterial Colonization Genetic Manipulation Symbiotic Bacteria Conditionally Essential Genes E. Coli Donor Burkholderia Gladioli Recipient Conjugation Mutant Library
Read Article
Waiting X
Simple Hit Counter