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JoVE Journal Biology
Quantification of Interbacterial Competition using Single-Cell Fluorescence Imaging
 

Quantification of Interbacterial Competition using Single-Cell Fluorescence Imaging

Article DOI: 10.3791/62851-v • 07:34 min • September 2nd, 2021
September 2nd, 2021 •
1, 1
1Department of Earth, Marine, and Environmental Sciences, University of North Carolina

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This manuscript describes a method for using single-cell fluorescence microscopy to visualize and quantify bacterial competition in coculture.

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Quantification Interbacterial Competition Single-cell Fluorescence Imaging Type VI Secretion System Bacterial Strains Total Cell Area Image Analysis Software Culturable Microbes Agarose Pad Solution Low Melt Agarose Marine PBS Upright Microscopes Inverted Microscopes Lab Tape Glass Slide Coverslip Width Agarose Pad Surface
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