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Imunologia e Infecção
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Primed Mycobacterial Uveitis (PMU) as a Model for Post-Infectious Uveitis
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Primed Mycobacterial Uveitis (PMU) as a Model for Post-Infectious Uveitis

Primed Mycobacterial Uveitis (PMU) as a Model for Post-Infectious Uveitis

DOI:
10.3791/62925-v

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10:33 min

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December 17, 2021

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, , , ,
1Department of Ophthalmology,University of Washington, 2Academic Unit of Ophthalmology, Translational Health Sciences,University of Bristol

Capítulos

  • 00:05Introduction
  • 01:26Antigen Preparation for Subcutaneous Injection
  • 02:31Subcutaneous Injection
  • 03:31Intravitreal Injection Procedure
  • 05:57Optical Coherence Tomography (OCT) Imaging to Detect and Quantify Uveitis
  • 07:35Results: Primed Mycobacterial Uveitis Model for Induction of Uveitis in Mice
  • 09:12Conclusion

Summary

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This protocol outlines the steps for inducing Primed Mycobacterial Uveitis (PMU) in mice. This method outlines the steps to help produce reliable and robust ocular inflammation in the mouse model system. Using this protocol, we generated uveitic eyes and uninflamed fellow eyes from single animals for further evaluation with immunologic, transcriptomic, and proteomic assays.

Tags

Primed Mycobacterial UveitisPMU ModelOcular InflammationChronic UveitisMycobacterial InfectionTransgenic MiceImmune PrivilegeHeat-killed MycobacteriaFreund’s Incomplete AdjuvantC57BL/6J MiceSubcutaneous Injection

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