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Journal / Immunology and Infection / Primed Mycobacterial Uveitis (PMU) as a Model for …
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Immunology and Infection

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JoVE Journal Immunology and Infection
Primed Mycobacterial Uveitis (PMU) as a Model for Post-Infectious Uveitis
 

Primed Mycobacterial Uveitis (PMU) as a Model for Post-Infectious Uveitis

Article DOI: 10.3791/62925-v • 10:33 min • December 17th, 2021
December 17th, 2021 •
1, 2, 1, 2, 1
1Department of Ophthalmology, University of Washington, 2Academic Unit of Ophthalmology, Translational Health Sciences, University of Bristol

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Summary
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This protocol outlines the steps for inducing Primed Mycobacterial Uveitis (PMU) in mice. This method outlines the steps to help produce reliable and robust ocular inflammation in the mouse model system. Using this protocol, we generated uveitic eyes and uninflamed fellow eyes from single animals for further evaluation with immunologic, transcriptomic, and proteomic assays.

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Keywords: Primed Mycobacterial Uveitis PMU Model Ocular Inflammation Chronic Uveitis Mycobacterial Infection Transgenic Mice Immune Privilege Heat-killed Mycobacteria Freund's Incomplete Adjuvant C57BL/6J Mice Subcutaneous Injection
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