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JoVE Journal Bioengineering
Microtubule Plus-End Dynamics Visualization in Huntington's Disease Model based on Human Primary Skin Fibroblasts
 

Microtubule Plus-End Dynamics Visualization in Huntington's Disease Model based on Human Primary Skin Fibroblasts

Article DOI: 10.3791/62963-v • 10:38 min • January 8th, 2022
January 8th, 2022 •
*1,2, *2,3, 1,2, 3,4, 3,4, 1,3
1A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 2Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, 3Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, 4Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency
* These authors contributed equally

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This protocol is dedicated to the microtubule plus-end visualization by EB3 protein transfection to study their dynamic properties in primary cell culture. The protocol was implemented on human primary skin fibroblasts obtained from Huntington's disease patients.

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Keywords: Microtubule Dynamics EB3 Protein Huntington's Disease Human Primary Skin Fibroblasts Cell Culture Transfection Fluorescent Labeling Microscopy
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