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Culture and Imaging of Human Nasal Epithelial Organoids
 

Culture and Imaging of Human Nasal Epithelial Organoids

Article DOI: 10.3791/63064-v • 13:20 min • December 17th, 2021
December 17th, 2021 •
1,2, 1,2, 1,2, 1,2
1Gregory Fleming James Cystic Fibrosis Research Center, University of Alabama at Birmingham (UAB), 2Department of Pediatrics, Division of Pulmonary and Sleep Medicine, UAB

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A detailed protocol is presented here to describe an in vitro organoid model from human nasal epithelial cells. The protocol has options for measurements requiring standard laboratory equipment, with additional possibilities for specialized equipment and software.

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Culture Imaging Human Nasal Epithelial Organoids Individualized Therapy Cystic Fibrosis CFTR Activity Lumens CF And Non-CF Organoids Culturing Imaging Methodology Dysfunctional Epithelial Ion Transport Chloride And Bicarbonate Epithelial Lining Fluids Mucus Secretions Mucostasis Obstruction HNE Model Investigator's Experimental Design And Resources Assessing CFTR Activity Therapeutics Diseases Involving Epithelial Cell Function Epithelial Cell Fluid Transport Cystic Fibrosis Studies Primary Ciliary Dyskinesia Studies Attention To Detail And Precision
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