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The Microscopy-Based Assay to Study and Analyze the Recycling Endosomes using SNARE Trafficking
 

The Microscopy-Based Assay to Study and Analyze the Recycling Endosomes using SNARE Trafficking

Article DOI: 10.3791/63087-v • 08:51 min • February 12th, 2022
February 12th, 2022 •
1, 1
1Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore KA 560012, India

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Recycling endosomes are part of the endosomal tubular network. Here we present a method to quantify the dynamics of recycling endosomes using GFP-STX13 as an organelle marker.

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Microscopy-based Assay Study Analyze Recycling Endosomes SNARE Trafficking Skin Melanocytes Melanosomes Lysozome Related Organelles Tubular Vesicular Organelles Biogenesis Cargo Premature Melanosomes Hyperpigmentation Dynamics Syntaxin-13 Seeding Mouse Melanocytes Coverslips Basement Membrane Matrix Medium PMA
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