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JoVE Journal Bioengineering
Control of Cell Adhesion using Hydrogel Patterning Techniques for Applications in Traction Force Microscopy
 

Control of Cell Adhesion using Hydrogel Patterning Techniques for Applications in Traction Force Microscopy

Article DOI: 10.3791/63121-v • 12:26 min • January 29th, 2022
January 29th, 2022 •
1, 2, 2, 1, 3, 3, 2, 1
1Department of Cellular Biophysics, Max Planck Institute for Medical Research, 2Institute for Theoretical Physics and BioQuant, Heidelberg University, 3Institute for Molecular Systems Engineering (IMSE), Heidelberg University

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Near-UV lithography and traction force microscopy are combined to measure cellular forces on micropatterned hydrogels. The targeted light-induced release of single cells enables a high number of measurements on the same sample.

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Hydrogel Patterning Techniques Cell Adhesion Control Traction Force Microscopy Methacrylated Glass Cover Slip Polyacrylamide Hydrogel Fluorescent Beads Micro-pattern Cover Slip Matrix Proteins Polymerize Fluorescence Microscopy Imaging Extracellular Matrix Cellular Traction Forces Light-induced Release Of Cells Experimental Observations Joel Christian Double Distilled Water Acetic Acid Trimethoxysilylpropyl Methacrylate Ethanol
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