Waiting
Processando Login

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / A Triple Culture Cell System Modeling the Human…
JoVE Journal
Biology

Este de .

JoVE Journal Biology
A Triple Culture Cell System Modeling the Human Blood-Brain Barrier
 

A Triple Culture Cell System Modeling the Human Blood-Brain Barrier

Article DOI: 10.3791/63134-v • 09:21 min • November 30th, 2021
November 30th, 2021 •
*1, *1, 1, 2, 3, 3, 3, 2, 1, 1, 1
1Univ. Artois, UR 2465, Laboratoire de la Barrière Hémato-Encéphalique (LBHE), 2Queen Mary University of London, Department of Chemistry, 3Yamaguchi University Graduate School of Medicine, Department of Neurology and Clinical Neuroscience
* These authors contributed equally

Capítulos

Resumo
Automatic Translation

This protocol describes a method to establish a human blood-brain barrier (BBB) in vitro model. The endothelial cells and pericytes are seeded on each side of an insert filter (blood compartment), and astrocytes are seeded in the bottom well (brain compartment). The model characterized was used for nanoparticles transport experiments.

Tags

Keywords: Blood-brain Barrier In Vitro Model Cell Culture System Astrocytes Cell-cell Communication Drug Delivery Preclinical Assessment Poly-L-Lysine Trypsin Astrocyte Medium Collagen Type 1
Read Article
Waiting X
Simple Hit Counter