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Biochemistry

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JoVE Journal Biochemistry
Efficient and Scalable Production of Full-length Human Huntingtin Variants in Mammalian Cells using a Transient Expression System
 

Efficient and Scalable Production of Full-length Human Huntingtin Variants in Mammalian Cells using a Transient Expression System

Article DOI: 10.3791/63190-v • 10:52 min • December 10th, 2021
December 10th, 2021 •
1, 1, 1, 1, 1, 1, 1
1Discovery Biology, Curia Global Inc.

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We provide scalable protocols covering construct design, transient transfection, and expression and purification of full-length human huntingtin protein variants in HEK293 cells.

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Full-length Huntingtin Variants Mammalian Cells Transient Expression System Low Milligram Scale High-quality Protein HD Researchers Michael Harris Curia Laboratory Polyethyleneimine 25 K Endotoxin Free-water PH Adjustment Hydrochloric Acid Sodium Hydroxide Solution 0.2-micrometer Filter HEK293 Cells Growth Medium Penicillin Streptomycin Shaker Incubator Carbon Dioxide Transfection Cell Density Viability Auto Cell Counter
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