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JoVE Journal Genetics
Optimized Bone Sampling Protocols for the Retrieval of Ancient DNA from Archaeological Remains
 

Optimized Bone Sampling Protocols for the Retrieval of Ancient DNA from Archaeological Remains

Article DOI: 10.3791/63250-v • 06:18 min • November 30th, 2021
November 30th, 2021 •
1,2, 1,3, 1,3, 1,3
1Department of Archaeogenetics, Max Planck Institute for the Science of Human History, 2School of Human Evolution and Social Change, Arizona State University, 3Department of Archaeogenetics, Max Planck Institute for Evolutionary Anthropology

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The protocol presents a series of best practice protocols for the collection of bone powder from eight recommended anatomical sampling locations (specific locations on a given skeletal element) across five different skeletal elements from medieval individuals (radiocarbon dated to a period of ca. 1040-1400 CE, calibrated 2-sigma range).

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Keywords: Ancient DNA Bone Sampling Skeletal Elements DNA Extraction Forensic Remains Non-human Specimens Dentin Petrous Pyramid Dental Drill Bone Powder Vertebral Arch Spinous Process Cortical Bone DNA Sampling Protocols
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