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Genetics

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JoVE Journal Genetics
Quantitative Real-Time Polymerase Chain Reaction Evaluation of MicroRNA Expression in Kidney and Serum of Mice with Age-Dependent Renal Impairment
 

Quantitative Real-Time Polymerase Chain Reaction Evaluation of MicroRNA Expression in Kidney and Serum of Mice with Age-Dependent Renal Impairment

Article DOI: 10.3791/63258-v • 06:48 min • April 29th, 2022
April 29th, 2022 •
1, 1, 1, 1,2, 3, 1
1Division of Nephrology, First Department of Integrated Medicine, Saitama Medical Center, Jichi Medical University, 2Division of Intensive Care Unit, First Department of Integrated Medicine, Saitama Medical Center, Jichi Medical University, 3Department of Medical Physiology, Meiji Pharmaceutical University

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We present a method for evaluating microRNA expression in the kidney and serum of mice with age-dependent renal impairment by quantitative reverse-transcription polymerase chain reaction.

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Keywords: QRT-PCR MicroRNA Expression Serum Age-dependent Renal Impairment RNA Extraction RNA Isolation Real-time PCR Gene Expression Profiling
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