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JoVE Journal Cancer Research
Subculture and Cryopreservation of Esophageal Adenocarcinoma Organoids
 

Subculture and Cryopreservation of Esophageal Adenocarcinoma Organoids: Pros and Cons for Single Cell Digestion

Article DOI: 10.3791/63281-v • 10:42 min • July 6th, 2022
July 6th, 2022 •
*1, *1, 1, 2, 1, 1
1Department of General, Visceral, Cancer and Transplantation Surgery, University Hospital Cologne, 2Institute of Pathology, University Hospital Cologne
* These authors contributed equally

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This protocol describes the methods of subculture and cryopreservation of esophageal adenocarcinoma organoids with and without single cell digestion to enable researchers to choose appropriate strategies based on their experimental design.

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Keywords: Esophageal Adenocarcinoma Organoids Subculture Single-cell Digestion PDOs ECM Gel Cell Recovery Solution Centrifugation Cryopreservation
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