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Isolation and Profiling of Human Primary Mesenteric Arterial Endothelial Cells at the Transcriptome Level
 

Isolation and Profiling of Human Primary Mesenteric Arterial Endothelial Cells at the Transcriptome Level

Article DOI: 10.3791/63307-v • 09:45 min • March 14th, 2022
March 14th, 2022 •
*1, *1, *1, 1,3, 2, 2, 1,3
1Department of Diabetes Complications and Metabolism, City of Hope, 2Department of Bioengineering, University of California San Diego, 3Irell and Manella Graduate School of Biological Sciences, City of Hope
* These authors contributed equally

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The protocol describes the isolation, culture, and profiling of endothelial cells from human mesenteric artery. Additionally, a method is provided to prepare human artery for spatial transcriptomics. Proteomics, transcriptomics, and functional assays can be performed on isolated cells. This protocol can be repurposed for any medium- or large-size artery.

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Keywords: Endothelial Cells Mesenteric Artery Transcriptome Single-cell Spatial Resolution Diabetes Obesity Cryostat Sectioning Cell Isolation Digestion Buffer Cell Culture
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