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Bioengineering

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JoVE Journal Bioengineering
Affordable Oxygen Microscopy-Assisted Biofabrication of Multicellular Spheroids
 

Affordable Oxygen Microscopy-Assisted Biofabrication of Multicellular Spheroids

Article DOI: 10.3791/63403-v • 13:21 min • April 6th, 2022
April 6th, 2022 •
*1, *1, 2, 3, 1
1Tissue Engineering and Biomaterials Group, Department of Human Structure and Repair, Faculty of Medical and Health Sciences, Ghent University, 2Health and Happiness (H&H) Group, National Food Innovation Hub, 3Institute of Analytical Chemistry and Food Chemistry, Graz University of Technology
* These authors contributed equally

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The protocol describes high-throughput spheroid generation for bioprinting using the multi-parametric analysis of their oxygenation and cell death on a standard fluorescence microscope. This approach can be applied to control the spheroids viability and perform standardization, which is important in modeling 3D tissue, tumor microenvironment, and successful (micro)tissue biofabrication.

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Affordable Oxygen Microscopy Biofabrication Multicellular Spheroids Fluorescence Microscope Oxygen Probe-stained Spheroid Microtissues 3D Printing Applications Vascularized Bone Tissue Grafts Near-infrared Oxygen Probe Green Fluorescence Staining Live Analysis Long-term Analysis PDMS Stamps Sterile Petri Dish Laminar Airflow Conditions Agarose Solution Sterile Spatula Sterile Water Cell Culture Media
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