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Immunology and Infection
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JoVE Journal Immunology and Infection
A Modified Surgical Technique for Kidney Transplantation in Mice
 

A Modified Surgical Technique for Kidney Transplantation in Mice

Article DOI: 10.3791/63434-v • 10:38 min • July 22nd, 2022
July 22nd, 2022 •
*1,2, *1,3, 4, 5, 1, 1, 6, 7, *5, *1
1Department of Pediatric and Adolescent Medicine, University Hospital Erlangen, Friedrich-Alexander University Erlangen-Nürnberg, 2Department of Pediatric Surgery, Chengdu Women’s and Children's Central Hospital, 3The Key Laboratory of Hainan Trauma and Disaster Rescue, The first affiliated Hospital of Hainan Medical University, 4Department of Department of Pediatric Kidney, Liver, and Metabolic Diseases, Hannover Medical School, 5Department of Nephrology, Hannover Medical School, 6Department of Nephrology, University Hospital Erlangen, Friedrich-Alexander University Erlangen-Nürnberg, 7Department of Dermatology, University Hospital Erlangen, Friedrich-Alexander University Erlangen-Nürnberg
* These authors contributed equally

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Resumo
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This protocol presents a new surgical technique of mouse kidney transplantation focusing on a modified arterial anastomosis strategy. A vascular suture technique including a simple and safer ureter-bladder anastomosis method is also presented. These modifications shorten the operation time and improve the success rate of the mouse kidney transplantation procedure.

Tags

Modified Surgical Technique Kidney Transplantation Mice Vessel Anastomosis Ureteral Implantation Abdominal Kidney Transplantation Model Aorta Ligation Renal Artery Renal Vein Transection Recipient Abdominal Aorta Anastomosis Ureteral Artery Preservation Anesthesia Isoflurane Inhalation Mouse Weight Intraperitoneal Injection Ketamine Xylazine Acepromazine Abdominal Fur Clipping Operation Table Fixation
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